Electron Microscopy Data Bank

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Cryo-EM subtomogram average of IFT complex B and inhibited dynein-1b in anterograde IFT trains (Chlamydomonas reinhardtii)

Release date: Oct. 31, 2018
Resolution: 37.0 Å
EM Method: Subtomogram averaging

Jordan MA, Diener DR, Stepanek L, Pigino G

Nat Cell Biol (2018) 20 pp. 1250-1255 [ DOI: doi:10.1038/s41556-018-0213-1 Pubmed: 30323187 ]

Sample:

Intraflagellar transport complex b and inhibited dynein-1b (Complex from Chlamydomonas reinhardtii)

EMD-4304

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Cryo-EM subtomogram average of IFT complex A in anterograde IFT trains (Chlamydomonas reinhardtii)

Release date: Oct. 31, 2018
Resolution: 33.0 Å
EM Method: Subtomogram averaging

Jordan MA, Diener DR, Stepanek L, Pigino G

Nat Cell Biol (2018) 20 pp. 1250-1255 [ DOI: doi:10.1038/s41556-018-0213-1 Pubmed: 30323187 ]

Sample:

Intraflagellar transport complex a (Complex from Chlamydomonas reinhardtii)

EMD-0161

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Retromer arch structures in the cell.

Release date: Sept. 26, 2018
Resolution: 37.0 Å
EM Method: Subtomogram averaging

Kovtun O, Leneva N, Bykov YS, Ariotti N, Teasdale RD, Schaffer M, Engel BD, Owen DJ, Briggs JAG, Collins BM

Nature (2018) 561 pp. 561-564 [ DOI: doi:10.1038/s41586-018-0526-z Pubmed: 30224749 ]

Sample:

Whole chlamydomonas cells (Cell from Chlamydomonas reinhardtii)

EMD-9618

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Localization of FAP70 N-terminus on Chlamydomonas axoneme

Release date: Sept. 12, 2018
Resolution: 40.0 Å
EM Method: Subtomogram averaging

Shamoto N, Narita K, Kubo T, Oda T, Takeda S

Cells (2018) 7 [ DOI: doi:10.3390/cells7090124 Pubmed: 30158508 ]

Sample:

Streptavidin labeled fap70::fap70-n-bccp axoneme (Cellular component from Chlamydomonas reinhardtii)

EMD-4332

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Segment of the cytoplasmic ring of the Chlamydomonas reinhardtii nuclear pore complex

Release date: July 4, 2018
Resolution: 30.0 Å
EM Method: Subtomogram averaging

Mosalaganti S, Kosinski J, Albert S, Schaffer M, Strenkert D, Salome PA, Merchant SS, Plitzko JM, Baumeister W, Engel BD, Beck M

Nat Commun (2018) 9 pp. 2361-2361 [ DOI: doi:10.1038/s41467-018-04739-y Pubmed: 29915221 ]

Sample:

EMD-4333

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Segment of the nuclear ring of the Chlamydomonas reinhardtii nuclear pore complex

Release date: July 4, 2018
Resolution: 30.0 Å
EM Method: Subtomogram averaging

Mosalaganti S, Kosinski J, Albert S, Schaffer M, Strenkert D, Salome PA, Merchant SS, Plitzko JM, Baumeister W, Engel BD, Beck M

Nat Commun (2018) 9 pp. 2361-2361 [ DOI: doi:10.1038/s41467-018-04739-y Pubmed: 29915221 ]

Sample:

Focus ion beam milled chlamydomonas cells (Cellular component from Chlamydomonas reinhardtii)

EMD-4334

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Segment of the spoke/inner ring of the Chlamydomonas reinhardtii nuclear pore complex

Release date: July 4, 2018
Resolution: 30.0 Å
EM Method: Subtomogram averaging

Mosalaganti S, Kosinski J, Albert S, Schaffer M, Strenkert D, Salome PA, Merchant SS, Plitzko JM, Baumeister W, Engel BD, Beck M

Nat Commun (2018) 9 pp. 2361-2361 [ DOI: doi:10.1038/s41467-018-04739-y Pubmed: 29915221 ]

Sample:

Focus ion beam milled chlamydomonas cells (Cellular component from Chlamydomonas reinhardtii)

EMD-4355

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Chlamydomonas reinhardtii nuclear pore complex

Release date: July 4, 2018
Resolution: 30.0 Å
EM Method: Subtomogram averaging

Mosalaganti S, Kosinski J, Albert S, Schaffer M, Strenkert D, Salome PA, Merchant SS, Plitzko JM, Baumeister W, Engel BD, Beck M

Nat Commun (2018) 9 pp. 2361-2361 [ DOI: doi:10.1038/s41467-018-04739-y Pubmed: 29915221 ]

Sample:

Focus ion beam milled chlamydomonas cells (Cellular component from Chlamydomonas reinhardtii)

EMD-9678

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Photosystem I of Chlamydomonas reinhardtii

Release date: June 20, 2018
Resolution: 2.89 Å
EM Method: Single-particle
Fitted PDBs: 6ijj
Q-score: 0.537

Su X, Ma J, Pan X, Zhao X, Chang W, Liu Z, Zhang X, Li M

Nat Plants (2019) 5 pp. 273-281 [ DOI: doi:10.1038/s41477-019-0380-5 Pubmed: 30850819 ]

Sample:

Psaf (24 kDa, Protein from Chlamydomonas reinhardtii)
Digalactosyl diacyl glycerol (dgdg) (949 Da, Ligand)
Psad (21 kDa, Protein from Chlamydomonas reinhardtii)
Chlamydomonas reinhardtii psi with eight light harvesting antennae (Complex from Chlamydomonas reinhardtii)
Psal (27 kDa, Protein from Chlamydomonas reinhardtii)
Dodecyl-alpha-d-maltoside (510 Da, Ligand)
Lhca6 (35 kDa, Protein from Chlamydomonas reinhardtii)
Lhca1 (26 kDa, Protein from Chlamydomonas reinhardtii)
Chlorophyll a (893 Da, Ligand)
Lhca3 (32 kDa, Protein from Chlamydomonas reinhardtii)
Psac (8 kDa, Protein from Chlamydomonas reinhardtii)
Lhca5 (30 kDa, Protein from Chlamydomonas reinhardtii)
Lhca4 (31 kDa, Protein from Chlamydomonas reinhardtii)
Psaa (83 kDa, Protein from Chlamydomonas reinhardtii)
(1r,3r)-6-{(3e,5e,7e,9e,11e,13e,15e,17e)-18-[(1s,4r,6r)-4-hydroxy-2,2,6-trimethyl-7-oxabicyclo[4.1.0]hept-1-yl]-3,7,12,16-tetramethyloctadeca-1,3,5,7,9,11,13,15,17-nonaenylidene}-1,5,5-trimethylcyclohexane-1,3-diol (600 Da, Ligand)
1,2-distearoyl-monogalactosyl-diglyceride (787 Da, Ligand)
Beta-carotene (536 Da, Ligand)
Iron/sulfur cluster (351 Da, Ligand)
Psaj (4 kDa, Protein from Chlamydomonas reinhardtii)
Lhca8 (29 kDa, Protein from Chlamydomonas reinhardtii)
Psai (10 kDa, Protein from Chlamydomonas reinhardtii)
1,2-dipalmitoyl-phosphatidyl-glycerole (722 Da, Ligand)
Phylloquinone (450 Da, Ligand)
Psae (16 kDa, Protein from Chlamydomonas reinhardtii)
(3r,3'r,6s)-4,5-didehydro-5,6-dihydro-beta,beta-carotene-3,3'-diol (568 Da, Ligand)
Lhca7 (26 kDa, Protein from Chlamydomonas reinhardtii)
Psab (82 kDa, Protein from Chlamydomonas reinhardtii)
Psak (16 kDa, Protein from Chlamydomonas reinhardtii)
(3s,5r,6s,3's,5'r,6's)-5,6,5',6'-diepoxy-5,6,5',6'- tetrahydro-beta,beta-carotene-3,3'-diol (600 Da, Ligand)

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EMD-9680

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Photosystem I of Chlamydomonas reinhardtii

Release date: June 20, 2018
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 6ijo
Q-score: 0.469

Su X, Ma J, Pan X, Zhao X, Chang W, Liu Z, Zhang X, Li M

Nat Plants (2019) 5 pp. 273-281 [ DOI: doi:10.1038/s41477-019-0380-5 Pubmed: 30850819 ]

Sample:

Lhca2 (24 kDa, Protein from Chlamydomonas reinhardtii)
Psaf (24 kDa, Protein from Chlamydomonas reinhardtii)
Digalactosyl diacyl glycerol (dgdg) (949 Da, Ligand)
Psal (27 kDa, Protein from Chlamydomonas reinhardtii)
Dodecyl-alpha-d-maltoside (510 Da, Ligand)
Lhca6 (35 kDa, Protein from Chlamydomonas reinhardtii)
Lhca9 (22 kDa, Protein from Chlamydomonas reinhardtii)
Lhca1 (26 kDa, Protein from Chlamydomonas reinhardtii)
Chlorophyll a (893 Da, Ligand)
Lhca3 (32 kDa, Protein from Chlamydomonas reinhardtii)
Psac (8 kDa, Protein from Chlamydomonas reinhardtii)
Lhca5 (30 kDa, Protein from Chlamydomonas reinhardtii)
Lhca4 (31 kDa, Protein from Chlamydomonas reinhardtii)
Psaa (83 kDa, Protein from Chlamydomonas reinhardtii)
(1r,3r)-6-{(3e,5e,7e,9e,11e,13e,15e,17e)-18-[(1s,4r,6r)-4-hydroxy-2,2,6-trimethyl-7-oxabicyclo[4.1.0]hept-1-yl]-3,7,12,16-tetramethyloctadeca-1,3,5,7,9,11,13,15,17-nonaenylidene}-1,5,5-trimethylcyclohexane-1,3-diol (600 Da, Ligand)
1,2-distearoyl-monogalactosyl-diglyceride (787 Da, Ligand)
Beta-carotene (536 Da, Ligand)
Psah (17 kDa, Protein from Chlamydomonas reinhardtii)
Psad (27 kDa, Protein from Chlamydomonas reinhardtii)
Chainx (1 kDa, Protein from Chlamydomonas reinhardtii)
Iron/sulfur cluster (351 Da, Ligand)
Psaj (4 kDa, Protein from Chlamydomonas reinhardtii)
Psag (16 kDa, Protein from Chlamydomonas reinhardtii)
Lhca8 (29 kDa, Protein from Chlamydomonas reinhardtii)
Psai (10 kDa, Protein from Chlamydomonas reinhardtii)
1,2-dipalmitoyl-phosphatidyl-glycerole (722 Da, Ligand)
Phylloquinone (450 Da, Ligand)
Chlamydomonas reinhardtii psi with ten light harvesting antennae (Complex from Chlamydomonas reinhardtii)
Psae (16 kDa, Protein from Chlamydomonas reinhardtii)
(3r,3'r,6s)-4,5-didehydro-5,6-dihydro-beta,beta-carotene-3,3'-diol (568 Da, Ligand)
Lhca7 (26 kDa, Protein from Chlamydomonas reinhardtii)
Psab (82 kDa, Protein from Chlamydomonas reinhardtii)
Psak (16 kDa, Protein from Chlamydomonas reinhardtii)
(3s,5r,6s,3's,5'r,6's)-5,6,5',6'-diepoxy-5,6,5',6'- tetrahydro-beta,beta-carotene-3,3'-diol (600 Da, Ligand)

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